Thin Layer Chromatography Analysis Of Analgesic Drugs

Experiment 2 Thin Layer Chromatography analysis of analgesic drugs Miten Patel Chemistry 3201 Date 2-2-2017 Objective: To Find the composition of a series of over the counter painkillers using TLC. Chemical Equation: N/A Chemical Mechanism: N/A Safety Considerations: †¢ All Students must wear goggles when dealing with the chemicals in the lab, and wear gloves to protect their skin from the chemicals used. †¢ The capillary tubes are fragile and sharp. For clean-up, the chemicals can be disposed of down the drain. Back ground Thin layer chromatography In this lab, we were working with pain reliever drugs. Which we need to identify by TLC method. In chromatography, a partitioning occurs between a mobile phase and†¦show more content†¦Which is going to be Aspirin and Acetaminophen. 3. Prepare a TLC chamber using a 250 ml clean and dry beaker. Then put a filter paper in beaker horizontally and add 15ml ethyl acetate containing 0.5% acetic acid. (The reason is put paper horizontally, when we put our alcohol in beaker so paper can stick with it). Cover the beaker. 4. Place the plate in TLC chamber and allow the solvent to run plate to about 1cm from the top. When solvent run above 1cm on plate take that plate out from chamber and drew a line with pencil. 5. Then see your plate in UV lights (short wavelength) and circle all what you see in UV lights. 6. Place your plate in iodine chamber for 2 to 3 minutes then make sure all your circle and calculate retention factor (Rf) value with the help of equation above. And stick plates in our notebook. Rf = Distance traveled by the sample (cm) Distance traveled by the solvent (cm) For unknown 7. I have no 54 unknown sample to identify which is in solid form. In the given sample add 50/50 mixture of ethanol and methylene chloride. Stair it and mix it well. Allow some time to mix it well so insoluble fraction can dissolved properly. 8. Spot the mixture by using capillary on TLC plate like we did on the first part. Prepare TLC chamber with filter paper and ethyl acetate. Put your TLC plate in chamber and allow it to run plate above 1cm at top. Mark the position. 9. Check your plate in UV lights. Circle all spot calculate the value of Rf with help ofShow MoreRelatedPRACTICAL REPORT ON THE ISOLATION AND IDENTIFICATION OF CODEINE AND PARACETAMOL1201 Words   |  5 Pagesidentify in comparison to standards using Thin Layer Chromatography. INTRODUCTION: Codeine or methyl morphine, an alkaloid, was first isolated in 1832 from raw opium. It concentration ranges from 0.2% to 0.8%. Mostly used for its analgesic, anti-tussive and anti-diarrheal capabilities (Tremlett, Anderson and Wolf, 2010). Paracetamol also known as acetaminophen (n-acetyl-p-aminophenol, APAP) on the other hand, is a useful non- steroidal anti- inflammatory drug (NSAID). 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